a) If a mutant grows well on YPD but fails to grow on the YPD+H2O2 plate, what c
ID: 63047 • Letter: A
Question
a) If a mutant grows well on YPD but fails to grow on the YPD+H2O2 plate, what can you predict about the function of the gene that is mutated with respect to the stress response and/or the respiratory pathway?
b) If a mutant grows well on YPD but grows even better on the YPD + H2O2 plate, what can you predict about the function of the gene that is mutated with respect to the stress response and/or the respiratory pathway?
c) If a mutant grows well on YPD but fails to grow on the YPG plate, what can you predict about the function of the gene that is mutated with respect to the stress response and/or respiratory pathway?
d) If a mutant grows well on YPD but grows even better on YPG plate, what acna you predict about the function of the gene that is mutated with respect to the stress response and/or the respiratory pathway?
e) If you explain growth on the YPD plate, provide at least two possible reasons that would explain not seeing growth on the YPD plate.
Explanation / Answer
a) This gene is producing peroxidase enzyme that eliminates the production of free radicles that formed during stress response and during electron transport chain.
b) This gene is producing a protein that makes use of H2O2 as their electron source.
c) This gene’s primary function is to produce carbohydrates from glycerol. As the mutant lack this functional gene, the bacteria is unable to utilize glycerol as carbohydrate source.
d) This gene can utilize glycerol as its carbohydrate source.
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