Aunt Jenny, age 110, is planning to prepare her patented fruit cake for the holi

Question

Aunt Jenny, age 110, is planning to prepare her patented fruit cake for the holidays, but she misplaced the family recipe and cannot remember what to add. Searching in the attic, she discovered the old baking pan that has not been used for the last 30 years. It has still some of the baked-on crust of a fruit cake stuck on the bottom. Aunt Jenny submits the crust to the local laboratory and has received a DNA sequence as result of their investigation. This sequence is available to you as SEQUENCE-1.fasta on MOODLE. It is your job to find out which fruits have been used in the old family recipe. Describe each step of your investigation

> Cake Sequence-1
GGATATTCTCCAGATAAAAGTCAGAATATGGTTCTTATGGGTGGTTTAAGAAAACATGTACCAATTACAA
AAACGTCTTTTTTATTAGGTACACTTTCTCTTTGTGGTATTCCACCTCTGGCTTGTTTTTGGTCCAAAGA
TGAAATTCTTAATGATAGTTGGTTGTATTCACCAATTTTTGCAATAATAGCTTATTCTACGGCAGGATTA
ACCGCTTTTTATATGTTTCGGATCTATTTACTTACTTTTGAAGGACATTTAAACGTTTATTTTCAAAATT
ACAGTGGCAAAAAAAGCAGCTCATTCTATTCAATGTCTCTATGGGGTAAAGAAGGACCTAAAATTATGAA
AAAAAACTTTCGTTTATTCGATTTATTAATGATGAAAAACAACGAAGGGGTTCCTTTTTTAAACACATAT
CGAATTGATAGTAATGAAAATGTAAGAAGCATGGTGCAGCCTTTTATTAGTATTACTCATTTTAGCACTA
AAAAAACTTTCTCATATCCCTATGAGTCGGACAACACTATGCTATTTCCCATGCTTGTATTGGTTTTATT
TACGTTATTCGTTGGGGCCGTAGGAATTCCTTTCTTCAATCAGGAAGGGGTGGGGTTAGATATATTATCC
AAATTATTAACTCCGTCCATAAACCTTTTACATCAAAACCGAACCCATTCTGTCGATTGGTATGAATTTC
TTATAAACGCAGTTTTTTCAGTCAGTATAGCTTATTGCGGAATACTTATAGCGTCCTTTTTATATAAGCC
TGTTTATTCATCTTTACAAAATTTGAATTTATTTAATTCATTTGTTAAAAGGGTTCCTAATAAAATGCAA
GTTTTGGGAGGGAAAATAATAAATGTGATATATGATTGGTCATATAATCGCGGTTACATAGATGTTTTTT
ATACAATCTCCTTAACTAAGTGTATAAGAAAATTAGCCGAATTAACTCATTTTTTTGATAGACGAGTAAT
CGATGGAATTACGAATGG TCTTTAAAAACTAAGGGGAGAGAGAGAGACAAAGAAAACAAACATGGATTC
CTCCAGATCCTTCACCGCAATGGCCCTCCTCTTGCTTTTCTCTCTCTTCGCGCTATCATCGGCGCTGGAC
ATGTCGATCATCGGCGAACTATCGAGTTCCAGGACCGACGACGAAGTGATGGCGATGTACGAGTCGTGGC
TAGTCAAGCACGGAAAGTCGTACAACGCTATCGGCGAGAAAGAGAAGAGGTTTCAGATCTTCAAGGACAA
CTTGCGGTTCATCGACGAGCACAACGCCGAGAGCCGGACCTACAAGGTCGGGTTGAACCGGTTCGCTGAC
CTGACCAACGACGAGTACCGGTCCATGTACTTGGGCGCTAGGACCGGATCCAGGCGCAGGCTTTCAACGC
AGAAGAGGAGCGACCGGTATGTTCCAGTCGCCGGCGAGAGCTTGCCGGACTCCGTCGATTGGAGAGAGAA
AGGAGCCGTCGTTGGGGTCAAAGACCAGGGGAGCTGTGGGAGTTGCTGGGCTTTTTCGACTATTGCTGCG
GTGGAAGGGATCAACCAGATAGTTACTGGGGATTTGATCTCGCTGTCAGAGCAGGAGCTGGTGGACTGCG
ACACATCTTACAATGAAGGTTGCAATGGTGGTCTCATGGACTATGCCTTTGAGTTCATCATCAAAAATGG
TGGAATTGACACTGAGGAAGATTACCCGTATAATGCTAGAGATGGCAGATGCGACCAATACAGGAAAAAT
GCCAAGGTTGTTACCATTGACGATTATGAAGATGTTCCTGTAAACAACGAACAGGCGTTGCAGAAGGCAG
TTGCAAATCAACCCGTGAGCGTTGCCATTGAAGCCAGTGGCATGGCATTCCAATTCTATGAATCGGGTGT
ATTCACAGGAAATTGTGGGACTGCACTAGACCACGGTGTCACTGCTGTTGGATACGGTACAGAAAACAGC
GTGGACTACTGGATTGTGAAGAACTCATGGGGTTCAAGCTGGGGAGAGAGTGGTTACATCAGGATGGAGC
GTAACACGGGTGCCACAGGCAAATGTGGAATCGCAGTGGAGCCCTCTTATCCTATCAAGACGAGTCAAAA
TCCCCCCAACCCCGGCCCATCTCCTCCATCTCCAATTAAGCCTCCAACTGTTTGTGATGATTACTATACT
TGTCCTGAGAGCAGCACTTGCTGCTGTGTCTATGAATATGGCAAGTATTGCTTTGCCTGGGGATGCTGCC
CGCTTGAAGGCGCCACCTGCTGTGATGACCATTACAGTTGCTGCCCCCACGATTATCCAATTTGTAACGT
TTATGCTGGCACCTGCTTAATGAGCAAGGACAATCCGTTGGGAGTGAAGGCAATGAAGCGCATTCAGGCG
AAGCCTCAGTGGGCATTTGCGAATGACGGGAAAAGAAGCAGCGCGTAGGAAAGCTTTTTGAGCTTTTGAA
CAGTGGATGAGGAATGCAGTTCGAGGGTGAGAGGATTATAGCATGCTTTTTACTTGGACTTATAATTCAG
TATACCTCATTTCCACCGGATCGTTGGACGGTCAATTGATGATTATTCAGCTACTTATTTGTGTATATAA
TAATATGCTGCTTTACATTTCTGTTCAGCCTGAAAGACAAAACACATTGTTGTCAGTTTCTAGTTTCTGC
TGGTTATGTATATACTGCTTTAAATTTCTGGTAATAGCGGATATTCTCCAGATAAAAGTCAGAATATGGT
TCTTATGGGCGGTTTAAGAAAACATGTACCAATTACAAAAACTTCTTTTTTATTAGGTACACTTTCTCTT
TGTGGTATTCCACCTCTTGCTTGTTTTTGGTCCAAAGATGAAATTCTTAATGATAGTTGGTTGTATTCAC
CTATTTTTGCAATAATAGCTTATTCTACGGCAGGATTAACCGCCTTTTATATGTTTCGGATCTATTTACT
TACTTTTGAAGGACATTTAAACGTTTATTTTCAAAATTACAGTGGCAAAAAAAACAGCTCATTCTATTCA
ATGTCTCTATGGGGTAAAGAAGGACCTAAAATTATGAAAACAAACTTTCGTTTATTCGATTTATTAATGA
TGAAAAACAACGAAAGGACTGCTTTTTTAAACACATATCGAATTGATAGTAATGAAAATGTAAGAAGCAT
GGTGCATCCTTTTATTAGTATTACTCATTTTGGCACTAAAAAAACTTTCTCATATCCCCATGAGTCGGAC
AATACTATGCTATTTCCCATGCTTGTATTGGTTTTATTTACGTTATTCGTTGGAACCGTAGGAATTCCTT
TCTTCAATCAGGAAGGAATAGATTTAGATATATTATCCAAATTGTTAACCCCGTCCATAAACCTTTTACA
TCAAAATCGAACCCACCCTGTCGATTGGTATGAATTTATCACAAATGCAGTTTTTTCAGTCAGTATAGCT
TATTGTGGAATACTTATAGCGTCCTTTTTATATAAGCCTGTTTATTCATCTTTACAAAATTTGAATTTAT
TTAATTCGTTTGTTAAAAGGGCTCCTAATAAAATGCAAGTTTTTGGGGGTAAAATAAGAAATGTGATATA
TGATTGGTCATATAATCGCGGTTACATAGATGTTTTTTATATAATATCCTTAACTAAGGGTATAAGAAGA
TTAGCTGAACTAACTCATTTTTTTGATAGACGAGTAATCGATGGAATTACGAATGGAGTAGGTAT

Explanation / Answer

1) Go to NCBI tool (http://www.ncbi.nlm.nih.gov/)

2) Select BLAST on popular resources

3) Then Choose nucleotide blast under Choose a BLAST program to run.

4) Then copy and paste the given FASTA, gene sequence in Enter accession number(s), gi(s), or FASTA sequence(s).

5) Then click on the BLAST button to run, it will take a few seconds to run.

6) You can get summary report, like:

7) Below that you can find a Graphic Summary

8) Then place cursor (mouse) on the 199 Blast Hits on the Query Sequence (any sequence, you want).

9) You can find name of plant and protein form this genes in the results box.

Results:

From this process I found it is an NADH dehydrogenase F gene of Fragaria vesca sub species. Fragaria vesca is commonly called European strawberry, woodland strawberry, Alpine strawberry or wild strawberry.

Therefore, fruits have been used in the old family recipe are “strawberries.”

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