1. For the diffusion and osmosis lab, you gathered data based on changes in the

Question

1. For the diffusion and osmosis lab, you gathered data based on changes in the mass of model cells. To design your experiment, you made educated assumptions about what would pass through the dialysis tubing membrane, and what would not. Using the knowledge you gained during the macromolecules lab, specifically about the reagents that can be used to test for the presence of biological molecules, give examples of how you could have tested these assumptions in relation to movement of starch, glucose and protein (e.g albumin). These examples do not need to relate to the specific experiment your group did, and need only to address one biological molecule per example. (9 points)

Explanation / Answer

Diffusion is a physical process where molecules move from a region of high concentration to a region of low concentration. Osmosis on the other hand is another physical process where net movement of solvent molecules takes place from a region of low solute to high solute concentration, in such a direction which tends to equalize the concentrations on both sides. This movement takes place across a semi-permeable membrane. Thus, the size of molecules is a limiting factor. Hence out of glucose, starch and a protein only glucose can move across a membarne and the rest will be debarred because of their large size. This hypothesis can be tested by keeping a semipermeable dialysis bag full of only water in seperate beakers of glucose, starch and albumin solutions. After a while the water inside the bag should be tested for the presence of glucose, starch or albumin. It will be seen that only the presence of glucose would be detected and not starch and albumin.

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