The sequence elements listed in the left column are important for gene expressio

Question

The sequence elements listed in the left column are important for gene expression (some only in eukaryotes). Complete the table. For “Sequence”, write in the actual conserved sequence (see example below). For “Position within the gene”, write in where the sequence element is found relative to the following gene features: promoter, coding exons, 5’ UTR, 3’ UTR or introns. Use words like upstream, downstream, 5’ and 3’ to specify position.

Name of the Sequence                 Sequence                         Position within the gene

Proximal Promoter

i.e. TATA

Start codon

5’ splice site

3’ splice site

Stop codon

Cleavage and Polyadenylation Sequence

GU-rich sequence (required for cleavage)

GU-rich

Poly A tail

Proximal Promoter

i.e. TATA

Start codon

5’ splice site

3’ splice site

Stop codon

Cleavage and Polyadenylation Sequence

GU-rich sequence (required for cleavage)

GU-rich

Poly A tail

Explanation / Answer

Name of the Sequence                 Sequence                         Position within the gene

Proximal Promoter

i.e. TATA

At the 5' end)

Start codon

UAG

5' end

5’ splice site

MAG/GTRAGT

5' end

3’ splice site

CAG/G

3' end

Stop codon

UGA, UAA, UAG

3' end

Cleavage and Polyadenylation Sequence

AAUAAA

3' end

GU-rich sequence (required for cleavage)

GU-rich

3' end

Poly A tail

AAAAAAAAA (long chain of adenine)

3' end

Proximal Promoter

i.e. TATA

At the 5' end)

Start codon

UAG

5' end

5’ splice site

MAG/GTRAGT

5' end

3’ splice site

CAG/G

3' end

Stop codon

UGA, UAA, UAG

3' end

Cleavage and Polyadenylation Sequence

AAUAAA

3' end

GU-rich sequence (required for cleavage)

GU-rich

3' end

Poly A tail

AAAAAAAAA (long chain of adenine)

3' end

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