You have just discovered a new enzyme that catalyzes the degradation of cellulos

Question

You have just discovered a new enzyme that catalyzes the degradation of cellulose to glucose. You hope to commercialize this enzyme ultimately to invent a process for biofuel manufacture. In order to determine how efficient the enzyme is and how fast the enzyme catalyzes the reaction, you have to perform some basic enzyme kinetics. Plot the following enzyme data for the enzyme catalyzed reaction and determine the Km and Vmax. Enzyme Data for the Enzyme Catalyzed Reaction Substrate (cellulose) Concentration (molar concentration) Rate of conversion from cellulose to glucose (m/min) 0 0 6 98 15 230 24 390 50 600 95 980 180 700

Explanation / Answer

Based on the given kinetic data,

The saturation curve is:

To calculate the LB plot we need to reciprocate the data,

The LB plot is:

Therefore,

The Vmax is:

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