genetic question I need help with these question 1. On a piece of paper draw two
ID: 64441 • Letter: G
Question
genetic question I need help with these question 1. On a piece of paper draw two line to represent the sense strand of a gene and its mature mRNA and label the following. a. 5' and 3' b. nucleotide #1 c. gyons and intervening sequences (introns) d. upstream and downstream e. core promoter I. TATA box g. proximal promoter h. CAAT. CACCC boxes i. GC rich region j. 5'UTR. 3'UTR k. translation initation codon l. translation stop (termination) Gorton m. enhancer n. silencer o. transcription stop/mRNA cleavage signal (AATAAA) P. CAP q. branch site r. poly A 2. What are the functions of CAP and poly A? 3. How does the cell know where to space pre-imRNA? 4. What role do snRNPs play in mRNA splicing? 5. What would be the effect of a mutation in the following? a. translation start codon (ATG)Explanation / Answer
2. Once the mRNA is transcribed from the DNA, it is subjected to the modifications on 5’ and 3’ end such as addition of polyAs on 3’ end and cap on 5’ end. Addition of 100-250 adenine(s) to the 3’ end of the mRNA is referred as polyadenylation and addition of GTP (guanosine triphosphate) on 5’ end of mRNA is referred as 5’ cap. Both the modifications prevent the degradation of mRNA by RNase and in addition, they serve as a signal that allows mRNA to leave the nucleus.
3. In the pre-mRNA, there is specific signature sequences that allows the spliceosome to recognize the introns. In an intron, GU at 5’ splice site, AG at 3’ splice site along with A (branch point) followed by a region rich in pyridimes allows the splicesome to recognize the introns in pre- mRNA and cleave them to form functional mRNA.
4. snRNP are small nuclear ribonucleoproteins. snRNP is a complex of RNA and proteins that form spliceosome machinery in eukaryotes which is known to facilitate chopping of introns from pre- mRNA in eukaryotes and subsequently result in formation of mRNA which can be translated to proteins.
5. A mutation in the start codon can prevent the assembly of translation machinery which can further prevent the initiation of protein synthesis.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.