Write your answer on the pages provided at the end of the examination. The kinet
ID: 64671 • Letter: W
Question
Write your answer on the pages provided at the end of the examination. The kinetic data for an enzyme reaction, in the presence and absence of inhibitors. are plotted in the figure below. What is the name for this plot? How should the axes be labelled? b. Line A corresponds to enzyme without inhibitor. Explain how you would determine the Km of the enzyme for its substrate, and its Vmax activity. c. Identify the curve that corresponds to the addition of a competitive inhibitor. an uncompetitive inhibitor. a noncompetitive inhibitor.Explanation / Answer
Based on the given data,
a)
The given plot is called reciprocal plot or LB plot. It is plotted taking 1/v on y-axis and 1/s on x-axis.
b)
The km and Vmax can be determined form the obtained regression equation of line A.
c)
i) A competitive inhibitor- B
ii) An uncompetitive inhibitor - C
iii) A non-competitive inhibitor – D
For example, consider following data,
The saturation curve is:
To calculate the LB plot we need to reciprocate the data,
The LB plot is:
Therefore,
The Vmax is:
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.