1. Match the function described with the enzyme. a) Makes a complementary strand
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Question
1. Match the function described with the enzyme.
a) Makes a complementary strand of RNA (DNA Pol I, primase,helicase, DNA Pol III, ligase, topoisomerase)
b) Allows DNA to unwind to reduce tension. (DNA Pol I, primase,helicase, DNA Pol III, ligase, topoisomerase)
c) Forms a phosphodiester bond between two Okazaki fragments. (DNA Pol I, primase,helicase, DNA Pol III, ligase, topoisomerase)
D) Removes primers (DNA Pol I, primase,helicase, DNA Pol III, ligase, topoisomerase)
2. A replication fork is shown below. The triangle with "H" represent helicase. The 5' and 3' ends of the two template strands are labeled. Do you have enough information to determine which strand (A or B) will be the template for leading strand synthesis?]
Select one:
a. Yes...strand A is the template for the leading strand.
b. Yes...strand B is the template for the leading strand.
c. No...more information is needed to determine which is the template for the leading strand.
3.
The image below is.... (You may select more than one answer)
Select one or more:
a. seen during mitosis but not during meiosis.
b. one chromosome that has been replicated.
c. two homologous chromosomes.
d. A pair of sister chromatids.
Explanation / Answer
1. a) Makes a complementary strand of RNA ---------> primase
Primase is an RNA polymerase that reads DNA and synthesize complememntary RNA strand (mRNA).
b) Allows DNA to unwind to reduce tension. -------> helicase (Helicases can unwind double stranded DNA)
c) Forms a phosphodiester bond between two Okazaki fragments. -----> ligase
DNA ligase is an enzyme, which seals the breaks and fills in gaps in a DNA strand during replication process.
D) Removes primers -----------------------> DNA Pol I (DNA polymerase I).
DNA polymerase removes primers by a process called nick translaton.
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