(a) The active site of an enzyme is shown by chemical labeling experiments to co

Question

(a) The active site of an enzyme is shown by chemical labeling experiments to contain a reactive lysine residue. Assuming that this is the only polar residue in that micro-environment, what would you expect to be the micro-pH of that active site pocket in the enzyme if this amino acid were 50% dissociated?

(b) Show the cleavage site for EcoR1 in the single-stranded DNA sequence below:

                5’ Gtcacctggaattcattgcgct 3’

(c) What role does topoisomerase play in mammalian cells?

(d) what is the overall mechanistic difference between topoisomerase I and II ?

(e) Why, in sequencing a protein, are the subunits first dissociated?

(f) Which is more likely to be able to form an amphipathic structure: an alpha helix OR a beta strand? Why?

Explanation / Answer

b)gaattc

C)it works during DNA replication in overwinding and underwinding of DNA

D)topoisomerasel:cits one strand of DNA double helix relaxation occurs and then cut strand is reannealed.cutting of one strand allow the part of the molecule on ine side of the cut to rotate around the uncut strand ,thereby reducing stress from too much or too little twist in the helix.

Topoisomerase ll:cuts both strand of one DNA double helix passes another unbroken DNA helix through it and then reanneals the cut strand.

f)alpha helix..due to deposition of hydrophobic and hydrophillic amino acid

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