SST1 Knockouts : IF YOU PLAGIARIZE OR PUT A RANDOM ANSWER, YOU WILL BE REPORTED.

Question

SST1 Knockouts : IF YOU PLAGIARIZE OR PUT A RANDOM ANSWER, YOU WILL BE REPORTED. ONLY ANSWER IF YOU ARE CONFIDENT.

The figure below diagrams the correct one-step disruption of the SST1 gene. The structure shown is what would be expected for digested disruption DNA. Nonhomologous insertion is also possible.

If you wished to use PCR to confirm the DNA structure of candidate sst1 knockout: Show on the diagram below the location of primer pair(s) you could use to distinguish correct knockouts, from random insertions, from no insertion at all. Also explain how your PCR reaction set would work:

Explanation / Answer

If you design the forward primer from 5' half end with start codon ATG and reverse primer from 3' half end of reverse complementary sequence would help in the complete amplification of URA3 non-inserted sst1 gene by PCR. If URA3 is inserted randomly in sst1 gene results in the disruption, so that primers cannot amplify once they reach to URA3 sequence ends in the disrupted sst1 gene.

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