How many nucleotides does a DNA probe need to only mismatch 119 times in a 2 bil

Question

How many nucleotides does a DNA probe need to only mismatch 119 times in a 2 billion base par air genome?

Explanation / Answer

Method Read length Accuracy (single read not consensus) Reads per run Time per run Cost per 1 million bases (in US$) Advantages Disadvantages Single-molecule real-time sequencing (Pacific Biosciences) 10,000 bp to 15,000 bp avg (14,000 bp N50); maximum read length >40,000 bases[61][62][63] 87% single-read accuracy[64] 50,000 per SMRT cell, or 500–1000 megabases[65][66] 30 minutes to 4 hours[67] $0.13–$0.60 Longest read length. Fast. Detects 4mC, 5mC, 6mA.[68] Moderate throughput. Equipment can be very expensive. Ion semiconductor (Ion Torrent sequencing) up to 400 bp 98% up to 80 million 2 hours $1 Less expensive equipment. Fast. Homopolymer errors. Pyrosequencing (454) 700 bp 99.9% 2 million 24 hours $10 Long read size. Fast. Runs are expensive. Homopolymer errors. Sequencing by synthesis (Illumina) 50 to 300 bp 99.9% (Phred30) up to 6 billion (TruSeq paired-end) 1 to 11 days, depending upon sequencer and specified read length[69] $0.05 to $0.15 Potential for high sequence yield, depending upon sequencer model and desired application. Equipment can be very expensive. Requires high concentrations of DNA. Sequencing by ligation (SOLiD sequencing) 50+35 or 50+50 bp 99.9% 1.2 to 1.4 billion 1 to 2 weeks $0.13 Low cost per base. Slower than other methods. Has issues sequencing palindromic sequences.[70] Chain termination (Sanger sequencing) 400 to 900 bp 99.9% N/A 20 minutes to 3 hours $2400 Long individual reads. Useful for many applications. More expensive and impractical for larger sequencing projects. This method also requires the time consuming step of plasmid cloning or PCR.

Related Questions

Navigate

• Browse (All)
• Browse H
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.