Answer using the following restriction endonucleases Agrobacterium metaphase I h

Question

Answer using the following

restriction endonucleases              Agrobacterium                    metaphase I         haploid

vector                                                          Ti plasmid                               anaphase I             incomplete dominance

plasmid                                                                        vir genes                                   prophase I             polygenic inheritance

sticky ends                                                shuttle                                      prophase II           allele

transformation                                      callus                                         metaphase II        homozygous dominant

transgenic                                                 cloning                                     anaphase II           homozygous recessive

PCR                                                               expression                              diploid                    heterozygote

phenotype                                                genotype                                 gamete                    particle bombardment

_______________________ A technique used to amplify a specific region of DNA.

_______________________ A tool used in molecular biology to cut a vector and the gene of interest in order to anneal the two pieces.

_______________________ When a bacterial cell takes up DNA (e.g. a plasmid).

_______________________ Undifferentiated plant tissue in culture.

_______________________ During this stage of meiosis, homologous chromosomes pair.

_______________________ During this stage of meiosis, sister chomatids separate.

Explanation / Answer

1. PCR  (polymerase chain reation) -------------> A technique used to amplify a specific region of DNA.

Polymerase chain reaction (PCR) is a biotechnological method to replicate DNA (deoxyribonucleic acid) in the laboratory, and multiple copies of the same sequence can be produced.

2. Restriction endonucleases  ------------> A tool used in molecular biology to cut a vector and the gene of interest in order to anneal the two pieces.

3. Transformation------------> When a bacterial cell takes up DNA (e.g. a plasmid).

Transformation is a type of vertical gene transfer that occurs between bacteria. The genes released by dead bacteria can be transferred to the live bacteria, and get integrated into its genome, or transformation may occur alsi by inserting the genome b means of a vector, which results in genetic recombination of the host.

4. Callus ---------> Undifferentiated plant tissue in culture.

Callus (of plant) is an unorganized parenchymal cells.

5. Prophase -I -------------> During this stage of meiosis, homologous chromosomes pair.

6. Anaphase II ----------------->During this stage of meiosis, sister chomatids separate.

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