A. You are in a lab that is studying this signaling pathway, and it is your job

Question

A. You are in a lab that is studying this signaling pathway, and it is your job to figure out which component of the G-protein functions to signal the mating response. To do this, you create several different mutant yeast cell lines (left column in the chart above) and evaluate which cell division process the cells use in the presence and absence of the ?- factor (right and left columns, respectively). Taking the chart above into consideration, which component(s) of the G-protein normally transmits the mating response signal to the downstream effector molecules?

B. Now that you know which component carries the mating response signal to downstream effector molecules, predict the proliferation type in the absence and presence of ?-factor for the following mutants. Complete the chart with your predictions for each condition described.
- An ?-subunit that can bind GTP but cannot hydrolyze it.
- An ? subunit that does not have the myristoyl anchor.
- An ? subunit that cannot bind to, and interact with, the activated GPCR.

Explanation / Answer

Absence of alpha factor               Presence of alpha factor

An -subunit that can bind GTP but cannot hydrolyze it.

Mating response

Mating response

An -subunit that does not have the myristoyl anchor.

Normal proliferation

Normal proliferation

An -subunit that cannot bind to, and interact with, the activated GPCR.

Normal proliferation

Normal proliferation

An -subunit that can bind GTP but cannot hydrolyze it.

Mating response

Mating response

An -subunit that does not have the myristoyl anchor.

Normal proliferation

Normal proliferation

An -subunit that cannot bind to, and interact with, the activated GPCR.

Normal proliferation

Normal proliferation

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