Using this best-fit standard line, estimate the concentration of your purified a
ID: 65824 • Letter: U
Question
Using this best-fit standard line, estimate the concentration of your purified and unpurified GFP protein. Show on your graph where these absorbance values intersect the Y-axis, the "best-fit" straight line, and the X-axis.
Protein Sample
Absorbance at 595 nm
0 mg/mL (Buffer)
.430
0.2 mg/mL
.512
0.4 mg/mL
.596
0.6 mg/mL
.724
0.8 mg/mL
.788
1.0 mg/mL
.815
1.2 mg/mL
.890
1.4 mg/mL
.915
1.6 mg/mL
.970
1.8 mg/mL
.990
2.0 mg/mL
1.035
Unpurified GEP
.510
Purified GEP
.520
Protein Sample
Absorbance at 595 nm
0 mg/mL (Buffer)
.430
0.2 mg/mL
.512
0.4 mg/mL
.596
0.6 mg/mL
.724
0.8 mg/mL
.788
1.0 mg/mL
.815
1.2 mg/mL
.890
1.4 mg/mL
.915
1.6 mg/mL
.970
1.8 mg/mL
.990
2.0 mg/mL
1.035
Unpurified GEP
.510
Purified GEP
.520
Explanation / Answer
The equation is
y=0.2974x+0.4903
Here,
y= concentration
x= absorbance
y= 0.2974*0.510+0.4903
= 0.642 mg/ml
Unpurified GEP = 0.642 mg.ml
Purified GEP
y= 0.2974*0.520+0.4903
= 0.644 mg/ml
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