1. In addition to human error, list as many different parameters that can vary f

Question

1. In addition to human error, list as many different parameters that can vary from on infection to the next that may influence a typical titer procedure.

2. Name at least three factors that would decrease the titer of a given phage prepartation.

3. Knowing what you know about your phage, what are your predictions about the yield that you could expect from a 10-plate harvest?

4.Nucleases are enzymes that degrade nucleic acids. If your genomic DNA has nucleic contamination, what type of pattern would you expect to see on the gel?

Explanation / Answer

1. Contamination, proper addition of top agar

2, A. The phages may not induce lytic cycle

    B. The concentration of phages that are in lytic cycle may be too low to form a plaque

4. The DNA forms a continuous smear in stead of discrete bands

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