propose a reason for why this experiment (in the picture) may not have worked.be

Question

propose a reason for why this experiment (in the picture) may not have worked.be sure to explain the answer please.

a lub to do A Sent and huwe Toru J lab has taken the genes encoding for Cdk and M-cyelin, expressed them separanely in bacteria urified large quantities of both proteins, and then added them together to form a pure M eyeln- gels be Cdk complex. All these purification st has run, and he is able to see a nice cyclin-Cdk complex forming. He decides to inject the purified M cyclin-Cdk complex into oocytes that are not undergoing mitosis (ike the experiment described in class). He sees no effect of injecting purified M cyclin-Cdk into oocytes. As a control, he also repeats the experiment described in class and injects mitotic extract into oocytes and finds that the oocytes assemble a mitotic spindle (as expected). Given what you know abo M cyclin-Cdk regulation, propose a reason for why his experiment may not have worked? Be sure to explain your answer. (3 points)

Explanation / Answer

Cyclins are a group of proteins which facilitate the transition of cells through various stages of the cell cycle. They act by activating the enzymes called cyclin-dependent kinases or Cdks.

Cyclins have been divided into different classes such as G1/S cyclins, M cyclins and so on. The cyclin-Cdk complexes have a specific role during different stages of the cell cycle. They work in series one after the other and help to accomplish DNA replication, followed by mitosis and cell division. The cyclin-Cdk complexes need to undergo phosphorylation by specific kinases to become completely active.

M cyclin-Cdk complex comes into picture during mitosis where it promotes the formation of the spindle apparatus.

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