Yeast fun When the S. cerevisiae genome was sequenced, only about 40% of its pre

Question

Yeast fun

When the S. cerevisiae genome was sequenced, only about 40% of its predicted genes had been previously identified in forward genetic screens. This left about 60% of predicted genes with no known function, leading some to dub the genes fun (function unknown) genes.

Part A

As an approach to understanding the function of a certain fun gene, you wish to create a loss-of-function allele. How will you accomplish this?

As an approach to understanding the function of a certain fun gene, you wish to create a loss-of-function allele. How will you accomplish this?

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Part B

You wish to know under what conditions the gene expressed. How will you ascertain such information?

You wish to know under what conditions the gene expressed. How will you ascertain such information?

random transposable element insertion treat with a chemical mutagen injection of dsRNA for RNA interference homologous recombination to create a knockout inversion and homologous recombination

Explanation / Answer

PART A:

4. homologous recombination to create a knockout.

To create a complete loss-of-function allele, that particular gene can be inactivated by knockout.

PART B:

b. transcriptional fusion with GFP gene

To monitor the gene expression patterns, GFP (green fluorescent protein) genes can be used.

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