The most common causative agent of bacterial sexually transmitted disease is Chl

Question

The most common causative agent of bacterial sexually transmitted disease is Chlamydia trachoma is. It is estimated that there are 90 million new cases worldwide per year, and it is also a leading cause of blindness. Chlamydia infects cells by first attaching to non-phagocyte eukaryotic cells, which is quickly followed by internalization. This internalization step is critical to the pathogen city of Chlamydia and is a potential target of therapeutics. As a new cell biologist you have identified a protein (Up1) that Chlamydia injects into non-phagocyte eukaryotic cells. You decide to knockout this protein to see if it impacts on bacterial uptake. After infection, you measure the expression/uptake of green fluorescent Chlamydia, both control and Up1 knockout (Up1 KO), in non-phagocytic cells. The following are your results:

Explanation / Answer

The given graph is as follows:

a)

In control the Up1 protein expression is 100%, whereas the UpK1 knockout has expression level up to 20%. Therefore, due to minimal expression of Up1 protein the UpK1 knockout cells are able to infect non-phagocytic cells.      

b)

The role of Up1 would be act as a channel or a connecting molecule of pathogen internalization. Initially, the Up1 protein detects the nonpathogenic cells and forms a connection with nonpathogenic cells. This will allow the internalization of bacteria.

    

c)

The Up1 protein has greatest effect with endosome forming proteins, like clathrin and other cytoskeletal proteins.     

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