You were given 20 ul of plasmid DNA to do one restriction digest. You adjust the

Question

You were given 20 ul of plasmid DNA to do one restriction digest. You adjust the pipette correctly and pipette 10 ul into your reaction mixture. You notice that you have actually used up the entire 20 ul of plasmid DNA. What happened? Question 1 options:

A) You did not push all the way down to the first stop when you were pipetting the plasmid.

B) You did not place the tip on your Pipetman into the liquid containing the 20 ul of plasmid DNA.

C) You pushed down to the second stop on your Pipetman when you were pipetting your plasmid into your reaction tube.

D) You pipetted your restriction buffer into the tube containing your plasmid DNA.

Explanation / Answer

D) You pipetted your restriction buffer into the tube containing your plasmid DNA.

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