The ampicillin added to the LB + AMP plates was at a concentration of 50 mu g/ml

Question

The ampicillin added to the LB + AMP plates was at a concentration of 50 mu g/ml. The molecular weight of ampicillin is 403.45. What is the molarity of the drug in the Petriplates? You need to make 250 mL of a 50 mM Cacl_2 solution? On your reagent shelf you have a calcium chloride dehydrate How much Cacl_2 is needed to make this solution? Show your work. You have an internship in a local pharmacy. A recent customer submitted a prescription for 150 mL of a 8.5% hydrocortisone cream. 'The pharmacist informs you that the pharmacy only stocks 4% and 10% hydrocortisone cream. The pharmacist is very busy with other customers, and she asks you to prepare the prescription. How would you do it? Show your work.

Explanation / Answer

1. Mole is a unit of measurement used to express amounts of a chemical substance. The concentration of a solution is mostly expressed by its molarity, which is the number of moles of the dissolved substance per litre of solution.

Molarity (M) = weight/molecular weight * volume/1000

Number of Moles of ampicillin present in the broth = 50* 10^-6/ 403.45* 1000 = 1.239 or approximately 1.23 moles.

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