First, click the \"Show Lactose Meter\" and \"Show Legend\" boxes. Next, add all
ID: 67596 • Letter: F
Question
First, click the "Show Lactose Meter" and "Show Legend" boxes. Next, add all of the components to the Lactose regulatory circuit, the lacI (repressor) promoter, the lacI gene, the lacZ (b-galactisudase) gene, the lac promoter and the lac operator (lacO)). With no lactose in the system, is beta-galactosidase being made (circle one)? What is the repressor doing when lattice is not present? Why do you think it is called a repressor? Click on the red button on the Lactose Injector to inject a lame amount of lactose into the system. Once a lame amount has been added, stop the addition and let the system come to its natural conclusion. What happens to the level of b-galactosidase when you inject lactose in the system. W hat happens to the level of Lac repressor (Lacl) when you inject lactose into the system? The mRNA and the protein disappear over time. Which stays around longer, the mRNA or the protein? Why?Explanation / Answer
1. When lactose is absent, beta galactosidase is not synthesized
2. In the absence of lactose, the repressor protein binds to the operator region of the lac operon. When it binds to the operator, it prevents the binding of the RNA polymerase to the promoter region.
3. The level of beta galactosidase increases and the level of repressor protein reduces.
4. The mRNA is destroyed as soon as its function is over. The protein however stays around for some time since it acts as an enzyme to break down lactose.
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