B) During severe liver damage, an enzyme (E1 of problem A) is released into the

Question

B) During severe liver damage, an enzyme (E1 of problem A) is released into the bloodstream. After severe exercise, a muscle enzyme, E3, that catalyzes the same reaction is released into the blood stream. E1 and E3 can be differentiated easily because they have different Km values. (The Km of the muscle enzyme is 2 X 10-5 M.) An assay of a blood sample of a patient gave the results given below. Is the patient suffering from blood disease, or has he simply been exercising strenuously? The patient arrived at the hospital unconscious, so you can’t ask him any questions.

                        [S] (M)                       v (moles/ml serum/min)

5.0 X 10-5                    43                                           

7.0 X 10-5                    57                                           

1.0 X 10-4                    75                                           

1.5 X 10-4                    100                                         

2 X 10-4                       120                                         

3 X 10-4                       150                                         

6 X 10-4                       200

How would you draw this graph?

Explanation / Answer

Well, there seems to be elevation of LDH5, which is due to liver disease and/or muscle damage.

Graph can be drawn by plotting [S] on x-axis and V on Y-axis.

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