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In a three-point cross between a flowering plant heterozygous at the following l

ID: 6806 • Letter: I

Question

In a three-point cross between a flowering plant heterozygous at the following loci LzlzGlglSusu and a homozygous recessive flowering plant (lzlzglglsusu) you obtain the gene combinations of the progeny frequencies below. You know the genes are all on the 3rd chromosome of the plants so there must be a fair amount of recombination taking place. Using these data, determine the gene in the middle, map the chromosomes in order and give distances between them. Now calculate the interference and coefficient of coincidence on the above data set and tell me what it means


Phenotype of Testcross Genotype of Gametes Number of Progeny
   Wildtype                      Lz Gl Su                   286
   Sugary                          Lz Gl su                      4
   Glossy, sugary                Lz gl su                      40
   Lazy, glossy, sugary        lz gl su                      272
   Lazy                              lz Gl Su                   33
   Lazy, sugary                   lz Gl su                   44
   Glossy                           Lz gl Su                   59
   Lazy, glossy                   lz gl Su                      2


In a three-point cross between a flowering plant heterozygous at the following loci LzlzGlglSusu and a homozygous recessive flowering plant (lzlzglglsusu) you obtain the gene combinations of the progeny frequencies below. You know the genes are all on the 3rd chromosome of the plants so there must be a fair amount of recombination taking place. Using these data, determine the gene in the middle, map the chromosomes in order and give distances between them. Now calculate the interference and coefficient of coincidence on the above data set and tell me what it means


Phenotype of Testcross Genotype of Gametes Number of Progeny
   Wildtype                      Lz Gl Su                   286
   Sugary                          Lz Gl su                      4
   Glossy, sugary                Lz gl su                      40
   Lazy, glossy, sugary        lz gl su                      272
   Lazy                              lz Gl Su                   33
   Lazy, sugary                   lz Gl su                   44
   Glossy                           Lz gl Su                   59
   Lazy, glossy                   lz gl Su                      2


Explanation / Answer

Given data is

Phenotype of Testcross   Genotype of Gametes Number of Progeny
   Wild type                          Lz Gl Su                             286
   Sugary                            Lz Gl su                                4
   Glossy, sugary                Lz gl su                                40
   Lazy, glossy, sugary        lz gl su                              272
   Lazy                                 lz Gl Su                               33
   Lazy, sugary                   lz Gl su                               44
   Glossy                            Lz gl Su                               59
   Lazy, glossy                    lz gl Su                                 2

Total progeny = 740

Recombinant frequency between Lz and Gl = 40 + 33+ 44+ 59 = 176 / 740 = 0.24

Recombinant frequency between Gl and Su = 4 + 44+ 59+ 2 = 109 / 740 = 0.14

Recombinant frequency between Lz and Su = 4+ 40+ 33+ 2 = 79 / 740 = 0.10

From above frequencies the map distances are

Lz----------Su---------------Gl

ß--0.10--àß----0.14---à

ß--------0.24-------------à

coefficient of coincidence = actual double recombinant frequency / expected double recombinant frequency.

Actual double recombinant frequency = 44 + 59 = 103

Expected double recombinant frequency = 176 / 740 * 109 / 740 = 0.035 = 25.9 per 740.

coefficient of coincidence = 103 / 25.9

                                          = 3.97

Interference = coefficient of coincidence – 1

                    = 3.97 – 1 = 2.97

From above calculations it shows that there is a strong cross over between the genes during meosis.

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