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Could you help me solve this question? I am completely stuck :(Thanks for your e

ID: 684158 • Letter: C

Question

Could you help me solve this question? I am completely stuck :(Thanks for your efforts in advance.

An unknown protein has been isolated in your laboratory. It wasdetermined
that this protein contains 172 amino acids and that it does notcontain
tryptophan or phenylalanine. You have been asked to determine thepossible
tyrosine content of the protein. You prepare a pure 50Msolution of the
protein, place it in a sample cell with a 1-cm path length, and youmeasure
the absorbance of this sample at 280 nm in a UV-VisibleSpectrophotometer.
The absorbance of the solution is 0.372. How many tyrosine residuesare
present in this protein? (Molar absorptivity = 1225M-1cm-1).

Explanation / Answer


You can use the values given and substitute into the equationA = bC to solve for concentration, which is theconcentration of tyrosines per concentration of protein that youused.
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