Given the results below for + transform Nutrient Agar + amp plate, what mistake
ID: 68423 • Letter: G
Question
Given the results below for + transform Nutrient Agar + amp plate, what mistake could have been made?
A.incubating the plates for less than the time specified
B.heat shocking the competent cells for too long
C. not sterilizing the L-shaped spreader
D.adding the wrong amount of competent cells
Given the results below for + transform Nutrient Agar + amp plate, what is NOT a mistake that could have been made? colonies_failed
A.not using the L-shaped spreader to distribute the bacteria evenly over the plate
B.keeping the competent cells on ice until heat shock
C.not adding the plasmid before heat shock
D.not heat shocking the competent cells
If a student accidentally plated his entire transformation on a Nutrient Agar plate, is there any way she could determine which of the colonies were transformed with the plasmid?
A.She could streak the colonies on Nutrient Agar + amp plates and see which ones grow.
B.She could make new cultures with the colonies in a Nutrient broth treated with ampicillin and see which cultures grow.
C.She could isolate and sequence DNA from several colonies to determine which ones are transformants.
D.All the answer choices are correct.
Why were there no colonies on the No DNA Nutrient Agar + amp plate?
A.The gene of interest was excised from the plasmid by bacterial mutation.
B.They were not transformed with a plasmid containing resistance to ampicillin.
C.There should have been colonies on the No DNA Nutrient Agar + amp plate. If you did not see any, there was an error in your results
D.The transformation process is not very efficient. This time it did not work.
Why were there less colonies on the + transform Nutrient Agar + amp plate than on the + transform Nutrient Agar plate when cells were plated from the same microtube of cells?
A.All of the answer choices are correct.
B.Transformation is not very efficient. Many cells did not take up the ampicillin resistance plasmid.
C.The uptake rate of the plasmid varies. Not all the bacteria were transformed with enough copies of the ampicillin resistance plasmid to neutralize the ampicillin.
D.The colonies closer to the antibiotic discs were killed, while the farther away colonies survived.
Which of the following is NOT a feasible technological use of transformation?
A.genetically engineering plants to undergo photosynthesis without sunlight
B.producing viral proteins for use in vaccines in bacterial cells
C.genetically engineering salmon by inserting new genes that make the fish grow faster
D.producing human growth hormone for treating genetic diseases that cause stunted growth
Explanation / Answer
1. There are colonies of fungus which indicates contamination. Improper sterilization of the spreader could be cause for contamination.
Hence, the correct answer is C. not sterilizing the L-shaped spreader
2. D.not heat shocking the competent cells
There is cell growth on the medium which eliminates the possibilit of not heat shocking the competent cells
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