Answer the following short answer questions: a. Which gene is amplified by PCR t

Question

Answer the following short answer questions:
a. Which gene is amplified by PCR to differentiate between males and females?

b. Which library consists of a DNA copy of the mRNA in a cell?
c. What is a pseudogene?
d. What is the selectable marker present in pUC plasmids?

e. Which of the following are potential restriction enzyme sites (Pick one)?
1. 5’-ggatgg 2. 5’-ccatgg 3. 5’-aagctt 4. 5’-gaattc

f) During an investigation of a break and enter police obtain a DNA sample with the following STR profile:
Amel (X,Y), FGA (42,50), THO1 (13,13), D21S11(28,30,33), D8S1179 (12,14)

i. What is unusual about this STR profile?

ii. What is the genetic make-up of this individual that might narrow down the search?

Explanation / Answer

a) The Amelogenin gene is used for sex determination because there is a separate version of amelogenin gene for X-chromosome and Y-chromosome.

b) A cDNA library consist of DNA copy of mRNA in a cell.

c) Genes which are dysfunctional or lost their ability to code for proteins are called as pseudogene

d)Selectable marker present in pUC plasmids is ampicillin resistance gene.

e) Answer-4 5'gaattc-restriction enzyme site for EcoRI also 3-5'aagctt- restriction site for Hind III

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.