The underlying principle of \"classical\" methods of DNA sequencing is to separa

Question

The underlying principle of "classical" methods of DNA sequencing is to separate, by size, nested sets of DNA molecules. There are two main steps involved: (1) running a PCR-like sequencing reaction and then (2) running the extension products through a gel matrix to separate them by size. The reagents used in the sequencing reaction are similar to those used in PCR, yet there are some important differences. What are the main reagents of a sequencing reaction?

-a single primer, template DNA, DNA polymerase, and a mix of ddNTPs and dNTPs at a 100:1 ratio -two primers, template DNA, DNA polymerase, and a mix of ddNTPs and dNTPs at a 100:1 ratio -two primers, template DNA, DNA polymerase, and a mix of ddNTPs and dNTPs at a 1:100 ratio -a single primer, template DNA, DNA polymerase, and mix of ddNTPs and dNTPs at a 1:100 ratio

Explanation / Answer

Reagents used in the sequencing reaction are: a single primer, template DNA, DNA polymerase, and mix of ddNTPs and dNTPs at a 1:100 ratio

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