can you help me briefly understand the concept as well please and thank you you

Question

can you help me briefly understand the concept as well please and thank you

you discovered a gene that enables organisms to accumulate gold in their tissues by concentrating trace amounts found in normal soil. you want to transfer this gene into a plant. order the steps below that would accomplish this (from 1-6)

a. transfer the recombinant Ti plasmidinto Agrobacterium tumefaciencs

b. Infect the plant with the Agrobacterium strain

c. Digest the gold gene and a Ti plasmid with the approproate restriction enzymes

d. Amplify the gold gene with PCR

e. Use a selectable marker to identify plant cells that have integrated the recombinant plasmid into their genome

f. Insert the gold gene into the Ti plasmid

Explanation / Answer

Order would be-

d. Amplify the gold gene with PCR

f. Insert the gold gene into the Ti plasmid

c. Digest the gold gene and a Ti plasmid with the approproate restriction enzymes

a. transfer the recombinant Ti plasmidinto Agrobacterium tumefaciencs

b. Infect the plant with the Agrobacterium strain

e. Use a selectable marker to identify plant cells that have integrated the recombinant plasmid into their genome

Explanation- We first isolate the gene from the microbe and amplify it with the help of PCR. Then we insert it Ti plasmid and with the help of restriction enzyme, we make a suitable plasmid. This is infected into Agrobacterium so as to infect it into the plant cells. To detect which plant cells took in the plasmid is done with the help of selectable markers.

Related Questions

Navigate

• Browse (All)
• Browse C
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.