1) The absorbance of a 20 mM sample reads 1.5 using a 1 cmcell. What is the mola
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Question
1) The absorbance of a 20 mM sample reads 1.5 using a 1 cmcell. What is the molar absorptivity of the sample. 2a) If the sample concentration is reduced by 20%, what is thenew absorbance obtained? 2b) If the sample cell in the question (2a) is doubled insize, what should the new absorbance reading for the samplebe. 1) The absorbance of a 20 mM sample reads 1.5 using a 1 cmcell. What is the molar absorptivity of the sample. 2a) If the sample concentration is reduced by 20%, what is thenew absorbance obtained? 2b) If the sample cell in the question (2a) is doubled insize, what should the new absorbance reading for the samplebe.Explanation / Answer
a) the given data is Concentration C =20mM Absorbance A= 1.5 lengthof the sample l =1cm the molar absorptivity of the sample = A /C l = 1.5 /20 mM *1cm 1mM=0.1 cm = 1.5 / 2 =0.75 b)If the sample concentration is reduced by 20% =20mM - 20/100mM =(20-0.2)mM =19.8mM the new absorbance A = C l =0.75 *19.8mM* 1cM 1mM=0.1 cm = 0.75 *(19.8/10) = 1.485 c) If the sample cell in the question (2a) is doubled insize that means l= 2cm the new absorbance reading for thesample be A = C l = 0.75 *19.8mM* 2 cm =0.75 *(19.8/10) *2 = 2.97Related Questions
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