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This quesiton is for biochemistry 311 A.) Using the Genetic Code, translate the

ID: 68924 • Letter: T

Question

This quesiton is for biochemistry 311

A.) Using the Genetic Code, translate the following mRNA sequence. First, identify the Met start codon, then label the SD sequence. Remember that not all of the sequence will be translated, only codons from the Met start codon to the stop codon.

5'-GCUGGCAGAUCUAGGAGGUAGGCAUGCGCAGGUUCUCUGAGUGGCUCCACCCUUGA-3' 3.

B.) Using the Genetic Code, calculate the number of possible mRNA sequences that could code for the following polypeptide sequence. Consider only the open reading frame (no Shine-Dalgarno or anything else, just the open reading frame from start codon to stop codon). Show your work: under each amino acid, list all the codons for that amino acid. Then show how you arrived at your answer.

Met Leu Arg Trp Pro Phe Val Stop

Explanation / Answer

GCUGGCAGAUCUAGGAGGUAGGCAUGCGCAGGUUCUCUGAGUGGCUCCACCCUUGA

K G G A T Q R T C A C L P P R S A S

LYS GLY GLY ALA THR GLN ARG THR CYS LEU PRO PRO ARG SER ALA SER

b) ATGGAAACGTTGGAGGCTAGGGGCACAAGGCCTCCCCGACCCCACGAAGTCGCCCTATCTACGCCG

AUGGAGACACUGGAAGCACGCGGAACCCGACCACCGCGACCACACGAAGUCGCACUUUCCACUCCU

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