Oxidase and O-F Glucose Click on the “T” icon and select the Oxidase Test from t

Question

Oxidase and O-F Glucose

Click on the “T” icon and select the Oxidase Test from the Help files. Read through the test material to familiarize yourself with how the test is performed. How does the method for performing this test differ from most other Virtual Lab tests?

Close the ID Matrix and open the Virtual Lab Report. Note the recording of the result you entered for the Oxidase test and mention of the number of microbes eliminated by this information. Was this test useful in your ID of the unknown? Explain.

Now we will take a look at the O-F glucose tests.

Select OF glucose broth from the Media list. Label the tube “OFglu”. What pH indicator is responsible for its green color?

Remove the OF glucose broth tube from the incubator and record the result. Remove the OF glucose broth with oil overlay tube from the incubator and record the result. What results did you record for these two tests? (Highlight)

OF Glucose: + -                 OF Glucose with Oil Overlay: + -

Based on these results, is the use of glucose by this unknown dependent on the presence of oxygen? (Highlight)

Yes                                         No

Which of these best describes this unknown? (Highlight)

Oxidizer

Fermenter

Oxidizer and Fermenter depending on presence of O2

Explanation / Answer

If fermentation has occurred, the entire tube without oil will turn yellow and with oil overlay it turn into entirely yellow.

OF Glucose: +                  OF Glucose with Oil Overlay: +

Based on these results, is the use of glucose by this unknown dependent on the presence of oxygen? (Highlight)

Yes                                        No

Which of these best describes this unknown? (Highlight)

Oxidizer

Fermenter

Oxidizer and Fermenter depending on presence of O2

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