For each of the following genotypes, indicate whether beta-galactosidase and per

Question

For each of the following genotypes, indicate whether beta-galactosidase and permease enzymes will be expressed in an inducible (I), non-inducible (N) or constitutive (C) fashion. For example, if a genotype is inducible for beta-gal but constitutive for permease, enter IC. P stands for promoter and is wildtype in all cases. You will have only 2 tries on each genotype.

7.11pt Answer 8. 11p Answer 9.11p I+P+O+Z+ Y Answer 10.[lpt Answer 11. [pt I(s) P+0+Z+Y+ Answer 12. [pt Answer 13. [pt I(s) P+ O(c) Z+ Y+ Answer 4. [lpt I+ P+ O(e) ZY Answer 15. [lpt Answer 16. [lpt Answer 17.[lpt Answer 18.[lpt Answer

Explanation / Answer

Inducible operon (I): Ina negative inducible operon a repressor binds to operator and inhibits transcription. When an inducer is present, it binds to repressor and causes conformational changes thus the operon is transcribed.

Constitutive genes (C): The genes that are always expressed are called constitutive genes.

Non-inducible operon (N): The operon containing inducer that is not able to interact with repressor is called non- inducible operon.

I+ P+ O+ Z+ Y+: IC

I+ P+ O+ Z- Y+: IC

I+ P+ O+ Z+ Y- : IC

I(S) P+ O+ Z+ Y+: IC

I+ P+ O(C) Z+ Y+: IC

I(S) P+ O(C) Z+ Y+: IC

I+ P+ O(C) Z+ Y- : IN

I+ P+ O+ Z+ Y+ /I+ P+ O+ Z- Y- : NC

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