Pre-lab questions: There are 6 pre-lab questions in this experiment-these questi

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Pre-lab questions: There are 6 pre-lab questions in this experiment-these questions are listed 1-6 below and must be submitted prior to the experiment. There are additional questions throughout the protocol (some in BOLD and boxed). Answer all of them, and use your answers to help draw your method diagrams. Be sure your responses to questions 7-20 are clearly labeled in your lab notebook. An assignment will be created in Blackboard for you to submit your pre-lab responses. 1. What wavelengths of light (UV, specific colors, infrared, X-ray, etc.) do you expect plants to use for photosynthesis? Which ones would make the most sense and why? 2. Which of the following molecules have “high-energy" electrons available for chemical work? a. stearic acid, a "C18" fatty acid b. CO2 c. polypeptide consisting of 3 amino acids d. glucose e. f. H2O 3. In the first part of the protocol, you will be using "differential centrifugation" to enrich chloroplasts. a. Based on what's happening in the protocol (Page 3), at the end of Step 5, in which "fraction" are the chloroplasts: supernatant or pellet? "fraction'" are the chloroplasts: supernatant or pellet? 4. What is the precise location of chlorophyll in a plant cell? b. Based on what's happening in the protocol (Page 3), at the end of Step 7, in which 5. Based on Task 2 Step 6, calculate the concentration of chlorophyll if the absorbance reading of the acetone extract = 0.65. Assume no additional dilution was needed in step 5. 6. Assume that the estimated volume of your chloroplast suspension 0.5 mL. Using your answer in #5, which method (see Task 2, Step 8) will you use to get the chloroplast suspension to a final concentration of 0.5 mg chlorophyll/mL? Suggested Plan To execute all the steps of this investigation during the lab period, you will need to be well organized and will need to split up the work. You will split your lab group into 2 teams of 1-2 people each, Team A and Team B. In the protocol below, you see steps in the procedures that are divided between the two teams. I also highly suggest that you find ways to expedite the work within in each team, like in a "factory line system where each member of a team performs the next task in line for each tube

Explanation / Answer

1.

Photosynthesis:

6CO2 + 12 H20------VISIBILE LIGHT------------------ C6H12O6 + 6O2 + 6H2O

Plant uses visible wavelength of light that chlorophyll molecules absorb . Maximum absorption is blue and red light ( 410-662 nm)

UV light causes high energy release which is harmful thus cannot be utilized for photosynthesis, X- ray too have harmful effect.

2.

Carbon of fatty acid ( e.g. palmitic acid has oxidation number -2 or -3 which is greater than oxidation number of glucose carbon which is -1,0 or +1, so high energy molecules are of fatty acid hence Answer a is correct.

3.

Chlorophyll are present in thylakoid sac of chloroplast. They are present in mesophyll cell.

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