If the 5 splice site sequence changed from 5-GUAAGU-3 to 5-GUAUGU-3, predict the

Question

If the 5 splice site sequence changed from 5-GUAAGU-3 to 5-GUAUGU-3, predict the effect of the sequence change on U1 binding and U6 snRNP binding in an in vitro protein-RNA binding assay.

-The mutation will likely completely disrupt binding of U1 to the 5 splice. The mutation will likely decrease binding of the U6 snRNP with the 5 splice site. -The mutation will likely completely disrupt binding of U1 to the 5 splice. The mutation will likely enhance binding of the U6 snRNP with the 5 splice site. -The mutation will likely cause a decrease in binding of U1 to the 5 splice site but not completely disrupt that binding. The mutation will likely enhance binding of the U6 snRNP with the 5 splice site. -The mutation will likely cause a decrease in binding of U1 to the 5 splice site but not completely disrupt that binding. The mutation will likely decrease binding (or even completely disrupt it) of the U6 snRNP with the 5 splice site.

Explanation / Answer

Since the conversion of sequence from 5-GUAAGU-3 to 5-GUAUGU-3 is a mutation resulted due to purine to pyrimidine transition, it will result in a silent mutation. The AGU is converted UGU (A to U transition). The codon AGU encodes for serine while the codon UGU encodes for cysteine. Both the amino acids are hydrophilic. U 1 to U6 is small nuclear ribonuclear proteins (SnRNPs) required for pre mRNA splicing reaction. The amino acid change at U1 position does not disrupt binding but decrease the binding at U1 position while the mutation enhance the binding of U6 snRNP since the hydrophilic amino acid provides binding site at U6 snRNP with 5` splice site. Hence, option 3 is correct.

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