On a 0.242-mm-diameter column with a 0602-pm-thick stationary phase, an unretain

Question

On a 0.242-mm-diameter column with a 0602-pm-thick stationary phase, an unretained solute passes through the column in 2.25 min, while the analyte requires 8.44 min. Calculate the phase ratio, for this column Incorrect The phase ratio for a column is calculated using the following equation, where r is the radius of the column, d is the diameter of the column, and dy is the stationary phase thickness. Remember to express the radius or diameter of the column and the film thickness in the same units. Number = 110.04054 Calculate the partition coefficient, K, for the analyte. 2d, 4d Number K0.267 Determine the retention time, t, of the analyte if a column of the same length with a 0.242-mm diameter and a 0.301-um-thick stationary phase of the same composition and at the same temperature is used for the separation instead Number 10.71 min Give Up & View Solution Previous TryAgain e Next Exit DEC 5 2

Explanation / Answer

For the given column separation data,

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phase ratio (beta) = d/2df

with,

d = 0.242 mm

df = 0.602 um = 0.000602 mm

we get,

phase ratio (beta) = 0.242/2 x 0.000602 = 201

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Partition coefficient for the analyte K,

K = analyte retention time/unretained solute retention time

   = (8.44 - 2.25)/2.25

   = 2.75

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Retention time for 0.301 um thick stationary phase

= 8.44 x 0.301/0.602

= 4.22 min

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