You have isolated several different temperature sensitive mutant yeast strains t

Question

You have isolated several different temperature sensitive mutant yeast strains that stop proliferating when shifted from 25 degrees C to 37 degrees C. you analyze these mutant strains at the two different temperatures using FACS analysis in which a machine sorts cells according to the amount of DNA they contain. at 25 degrees C the FACS plots for all of the strains look like this: (picture)

after the mutant cell cultures gave been grown at 37 degrees C you again analyze

these cells using FACS. the plots in the picture represent the different plot types from mutant yeast strains.

for each of the following temperature sensitive mutations predict which FACS plot (A,B, or C) you would expect. circle the letter.

the plots and predictions are in the pictures. please circle the correct answer for each.

- 1. You have isolated several different temperature semsitive matant yeast strairs that stop proliferating when shifted from 25°C to 37 C, You analyae these mutant struins at the two different temperucures using FACS analysis in which a machine sorts cells according to the amount of DNA they coetain. At 25'C the FACS plots for all of the strains look lke this: Amount olf After the mutant cell cultures have been grown at 37°'C you again analyze these cells using FACS. The plots below represent the different plot ypes from mutant yeast sarwins of of of 2N Amount of Amount of Amount of

Explanation / Answer

An inactivating mutation in M-cyclin - A

A mutation that prevented initiation of replication – A

A mutation that prevented the dephosphorylation of S-Cdk – C

An inactivating mutation in the anaphase promoting complex (APC) – B

A mutation that stopped production of ATP – A

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