Suppose that a virulent form of pneumonia has appeared in North America and is s
ID: 69554 • Letter: S
Question
Suppose that a virulent form of pneumonia has appeared in North America and is spreading rapidly among young adults. A previously unknown bacterium has been isolated from several patients and is the suspected cause of the illness. The table below lists research techniques to begin a genetic characterization of the bacterium.Select the research techniques that can be used to study the GC content, genome size, and the number of chromosomes comprising the bacterial genome.
Suppose that a vinudent form of pneumonia has appeared in North America and is spreading rapidly amog suspected cause of the illness. The table below lists research techniques to begin a genetic characterization of the bacterium. Research method Description and use density gradient centrifugation DNA molecules are centrifuged on a CsCl gradient, separating the molecules by density and allowing characterization of D composition Thousands of known DNA fragments are affixed to a glass slide at known locations and probed with sample DNA or RNA to detect the presence of specific sequences or to compare gene expression in two different conditions DNA microarray analysis Fluorescent probes designed to match a specific sequence are used to detect and localize the presence of the target sequence on chromosomes. fluorescence in situ hybridization (FISH) polyacrylamide gel electrophoresis (PAGE) DNA molecules move with an electrical radient through a porous gel, which allows aster movement of smaller molecules. DNA fragments between 10-3000 bp are separated by size. PAGE is typically preceded by enzymatic digestion to break down larger DNA molecules pulse-field gel electrophoresis (PFGE) DNA molecules move through a gel with an electrical field, in which the voltage is Continued constantly switched in three directions. PFGE blow separates DNA ranging in size from kilobases to megabases sedimentation- velocity centrifugation UV absorption is used to observe movement of Hint O Previous Give Up & View Solution Check Answer 0 Next Exit
Explanation / Answer
1.
Research method
Description
Pulsed-field gel electrophoresis
DNA molecules move through a gel with an electrical field, in which the voltage is constantly switched in three directions. PFGE separates DNA ranging in size from kilobases to megabases.
Sedimentation-velocity centrifugation
UV absorption is used to observe movement of DNA during centrifugation to determine the velocity of sedimentation of the DNA sample. Mass and shape determine movement of the molecule, allowing molecular weight calculations.
Density gradient centrifugation
DNA molecules are centrifuged on a CsCl gradient, separating the molecules by density and allowing characterization of DNA composition.
2. What is the genetic material of the virus
3. Is the genome single stranded or double stranded.
Research method
Description
Pulsed-field gel electrophoresis
DNA molecules move through a gel with an electrical field, in which the voltage is constantly switched in three directions. PFGE separates DNA ranging in size from kilobases to megabases.
Sedimentation-velocity centrifugation
UV absorption is used to observe movement of DNA during centrifugation to determine the velocity of sedimentation of the DNA sample. Mass and shape determine movement of the molecule, allowing molecular weight calculations.
Density gradient centrifugation
DNA molecules are centrifuged on a CsCl gradient, separating the molecules by density and allowing characterization of DNA composition.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.