18. (6 pts) Cloning strains of E.coli come with or without a functional copy of

Question

18. (6 pts) Cloning strains of E.coli come with or without a functional copy of the gene RecA. Which one of the following two situations would you want to make sure you are using a strain that carries a functional copy of RecA, and why? [Note -just guessing one answer or the other without giving a reason is not enough to answer this question]. a) You are doing an experiment where you want to insert a copy of foreign DNA into the E.coli genome b) You are transforming E.coli with an inducible expression vector containing a large DNA insert.

Explanation / Answer

Option A - Doing an experiment where you want to insert a copy of foreign DNA into the E.coli genome - Will help to identify the presence of RecA gene. If the experiment results in production of recombinant gene it means the strain contains RecA gene or else it does not contain. Since Rec A is the protein which helps in recombination of genes between homologous chromosomes.

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.