Multiple choice questions, I need the CORRECT ANSWERS, 3500 points 1.The promote
ID: 69699 • Letter: M
Question
Multiple choice questions, I need the CORRECT ANSWERS, 3500 points
1.The promoter is in region_____________.
a.1
b.2
c.3
d.4
e.5
2.The stop codon is identified in region __________
a.4
b.5
c.6
d.7
e.8
3.A mutation is region 8 would be expected to:
a.disrupt transcription
b.disrupt ribosome
c.disrupt DNA polymerase binding
d.disrupt splicing
e.none of the above
4.Which is responsible for the transcription of structural genes in eukaryotes?
a.RNA polymerase I
b.RNA polymerase II
c.RNA polymerase III
d.DNA polymerase
E.RNA Synthesis
5.The eukaryotic TATA box is directly bound by a basal transcription factor known as:
a.TBF
b.TBP
c.TAF
D.TPP
E.TAT
6.mOST TRANSCRIPTIONAL ACTIVATOR proteins in eukarypotes function in the cell as:
a.monomers
b.dimers
c.trimers
d.tetramers
e.pentamers
7.The basal level of transcription of eukaryotic genes is quite high in vitro systems. This is because:
a.activation are present
b.formation of chromatin dos not occur in vitro
c.basal transcription factors are present
d.a and c
e.none of the above
8.Transcriptional repression by methylation of DNA involves:
a.methylation of adenine (A) bases of the sequence GATC
b.methylation of cytosine (C) bases of th sequence GATC
c.mthylation of cytosin (C) bases in CpG nuclotides
d.Methylation of guanosine (G) bases in CpG nucleotides
e.acetylation of hisone proteins
9.A eukaryotic DNA sequence that can increase the level of transcription is called:
a. a transcription factor
b.a transcritpional activator
c.TATA box
d.an enhancer
e.a co-activator
10.Which is generally true of eukaryotic transcriptional regulation?
a.activators and reprssors bind to the promoter while basal factors bind to the enhances
b.basal factors bind to the promoter, while activators bind to the enhancers
c.activators bind to the promoter while basal factors and rprssors bind to th enhancers
d.repressors bind to the promoter whil basal factors and activators bind to the enhancers
e.activators and basal factors bind to the promtoer while reprssors bind to the enhancers
11.You wish to sequence the promoters region of the mouse gene rab.To do this, you need to construct a library and then search for the gene sequence.You have a DNA probe based on the sequence of the protein coding region.Which of th follwoing strategies would you follow in order to carry this out?
A.construct a genomic library and screen for the promoter using PCR
b.construct a cDNA library and screen for the promotoer using PCR
c.construct a genomic library and screen for the promoter using PCR
d.Construct a cDNA library and screen for the promoter using colony hybridization
e.all of the above
12.if one were to examine th clones in a cDNA library derived from th heart muscle cells and the clones in a cDNA library derivd from prancreatic cells, you would find that:
a.the two libraries would hav the same cloned sequences
b.the two libraries would have completely different clone sequences from one another
c.the two libraries would share some common clond sequences, but would each have some sequences unique to that library, not to be found in the other
d.the sequences from both would also b the same as those found in the genomic library
e.none of th above
13.In a particular population at Hardy-Weinberg equilibrium, the frequency of recssive allele b for a particular gne is 0.01.Assuming that there are only two alleles for the gene, what would b th expected frequency for dominant allele B in th next generation?
a.0.01
b.0.02
c.0.98
d.0.99
e.none of the above
14.White wool in the sheep is controlled by a dominant allele R, and black wool by the recessive allele r.In an isolated popualtion of 6530 sheep, 514 are balck, 4981 are hterozygous, 1035 ar homozygous white. When will this population be expected to reach Hardy-Weinberg equilibrium?
a.in the next generation
b.in two generations
c.it already is in equilibrium
d.impossible to tell
e.it will not reach equilibrium
15.A population has th following genotypic disturbutions:
A^M A^M=1787, A^M A^O=2011, A^OA^O=2331.Is this population at H-W equilibrium?
a.yes, with allele frequencies of p=0.45562 and q=0.54437
b.yes, with frequencies of p=0.54437 and q=0.37862
c.no, with allele frequencies of p=0.45562 and q=0.37862
d.no, with xpectd genotypic ratios of p^2=1272, 2pq=3040, and q^2=1826
e.yes, with expctd genotypic frequencies of p^2=1778, 2pq=2015, and q^2=2336
16.The result of the Luria-Delbruck fluctuation expriment indicatd that:
a. most bacteria are naturally resistant to phage
b.a low level of bacteria become resistant to phage by mutation upon exposure to phage
c.most bacteria become resistant to phage by mutation upon exposure to phag
d.bacteria become resistant to phag by random spontaneous mutation
e.bacteria become resistant to phag by acquiring resistant traists upon repeated exposure
17.DNA probes are used for:
a.restriction ndonuclease analysis
b.sequencing
c.PCR
d.DNA library analysis
e.all of th above
18.Colony hybridization is used to:
a.isloate a gne of interest froma DNA library
b.analyze the presence or number of copies of a particular DNA segment in a chromosome
c.determine the size of a gene or sequencing of interest
d.replace the more tediuos method of library screening.
e.all of the above
19.For the colony hybridization procedure, which of the following is true:
a.individual colonies are isolated and analyzed by DNA sequencing
b.the DNA fragments are separated by size prior to transfer onto nitrocellulose membrane
c.the plasmid DNA must be denatured before hybridization
d.the relative positions of DNA fragments on the agarose gel are preserved after transfer to the nitrocllulose membrane
e.the cloned target sequence is detected by staining with thidium bromide
20.The following strand of DNA is to be used for sequencing 3'CGACTGACTCGACGGCCTAGCTATAG.The primer is GCTGACTG.The number of DNA fragments resulting from the ddCTP tube is expectd to be:
a.7
b.5
c.8
d.4
e.6
21.Which of the following is not true of heterozygosity for a gene deletion?
a.it is often detrimental if there is a gene dosage effect.
b.as long as one wildtype copy of any deleted gene is presnt on the homologous chromosome, no phenotypic consequnces will result.
c.it could be detected by Southern blot analysis
d.it could be detected by PCR analysis
e.none of the above
22.Robertsonian translocation arise from:
a.breaks at or near the chromosome of two acrocentric chromosomes
b.breaks at or near th centromeres of two metacentric chromosomes
c.breaks at or near th centromeres of one acrocentric and one metacentric chromosome
d.breaks in a large and a small chromosomes
e.transposable elements
Explanation / Answer
4. Which is responsible for the transcription of structural genes in eukaryotes?
b. RNA polymerase II
5.The eukaryotic TATA box is directly bound by a basal transcription factor known as:
b.TBP
6. MOST TRANSCRIPTIONAL ACTIVATOR proteins in eukaryotes function in the cell as:
c.trimers
7. The basal level of transcription of eukaryotic genes is quite high in vitro systems. This is because:
d. a and c
8. Transcriptional repression by methylation of DNA involves:
c. methylation of cytosin (C) bases in CpG nuclotides
9. A eukaryotic DNA sequence that can increase the level of transcription is called:
b. a transcriptional activator
10. Which is generally true of eukaryotic transcriptional regulation?
b. basal factors bind to the promoter, while activators bind to the enhancers
11.You wish to sequence the promoters region of the mouse gene rab. To do this, you need to construct a library and then search for the gene sequence. You have a DNA probe based on the sequence of the protein coding region. Which of the following strategies would you follow in order to carry this out?
A. construct a genomic library and screen for the promoter using PCR
12. if one were to examine the clones in a cDNA library derived from th heart muscle cells and the clones in a cDNA library derived from prancreatic cells, you would find that:
c. the two libraries would share some common cloned sequences, but would each have some sequences unique to that library, not to be found in the other
13.In a particular population at Hardy-Weinberg equilibrium, the frequency of recessive allele b for a particular gene is 0.01.Assuming that there are only two alleles for the gene, what would b the expected frequency for dominant allele B in the next generation?
d.0.99
16. The result of the Luria-Delbruck fluctuation experiment indicated that:
d. bacteria become resistant to phage by random spontaneous mutation
17. DNA probes are used for:
e. all of the above
18. Colony hybridization is used to:
a. isolate a gene of interest from a DNA library
19.For the colony hybridization procedure, which of the following is true:
a. individual colonies are isolated and analyzed by DNA sequencing
20.The following strand of DNA is to be used for sequencing 3'CGACTGACTCGACGGCCTAGCTATAG.The primer is GCTGACTG. The number of DNA fragments resulting from the ddCTP tube is expected to be:
a. 7 (The primer stops wherever C is present, hence there will be 7 fragments.
21. Which of the following is not true of heterozygosity for a gene deletion?
b. as long as one wild type copy of any deleted gene is present on the homologous chromosome, no phenotypic consequences will result.
22. Robertsonian translocation arise from:
a. breaks at or near the chromosome of two acrocentric chromosomes
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