Formulas and constants: G··-RI\' In k\'eq G\",--n 2AH\', Feel free to state your

Question

Formulas and constants: G··-RI' In k'eq G",--n 2AH', Feel free to state your assumptions if they will mak 5 points per multiple choice question (one best answer). ..RT Keq = e R = 831 5 J/mol·K 3: 9648 KJ/v-mol e a difference in how you answer a question. Choose the fact about buffers that is completely true. a) The pH of a buffered solution remains the same no matter how much acid or base is added. b) The strongest buffers are made of a strong base and a strong acid. c) To choose the buffering range for a buffer, take the average of all the pKas d) To choose the buffering range, find the pH equal to the pKas of one component ± 1 pH unit. e) The best pH for a biological buffer is always pH 7 ± 1 pH unit 2 These 4 amino acids are added to a cation exchange column (where components have t opportunity to replace a positive ion on the column) at pH 5: Which amino acid(s) will bind most strongly? ARG, ASN, GLU, THR a) GLU b) ASN and THR c) ARG d) THR e) ASN 3. calculate the turnover number of an enzyme, you need to know b) km for the substrate d) enzyme concentration a) initial velocity of the catalyzed reaction at low IS c) initial velocity of the catalyzed reaction at IS>km e) values for both C and D 4 Which of these statements about allosteric enzymes is always false? a) Allosteric enzymes generally contain several subunits b) Affinity for the T form is greater than affinity for the R form. c) The graph of an allosteric enzyme is always sigmoidal d) The substrate and inhibitors must be different compounds e) These enzymes have both activators and inhibitors. 5. (5 points) Schostosoma mansoni is a parasite that infects about 125 million people worldwide The infective larval form lives in lakes and rivers. Even though it cannot bite and it is too big to get through the skin's pores, the organism quickly penetrates human skin. Provide a reasonable explanation for how the larva enters through human skin.

Explanation / Answer

Biochemistry

multiple choice

1. The correct statement regarding buffer solutions are,

c. pKa should be chosen closer to pH of buffer

2. When the given amino acids are passed through a cation exchange column, the amino acid which will bind most strongly having highest +ve charge at pH 5 is,

c) ARG

3. To calculate turnover number for an enzyme we need to know,

e) Both C and D

5. False statement regarding allosteroc enzymes would be

b) Binding to T greater than R

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