1) Cation exchange chromatography, more specifically, uses a negatively charged
ID: 699594 • Letter: 1
Question
1)
Cation exchange chromatography, more specifically, uses a negatively charged ion exchange resin with an affinity for molecules having net positive surface charges.
therefore, at low pH=5, Lysine carries a positive charge on the side chain -NH2 group due to low pH but aspartic acid side chain -COOH remains uncharged.
thus Lysine takes more time in the stationary phase and separate in the last.
therefore,If a mixture of lysine and aspartic acid is applied to a cation exchange column at a low pH, the first amino acid to elute would be ASPARTIC ACID.
2)
Examples of common glycoaminoglycansare chondroitin 6-sulfate, keratan sulfate, heparin, dermatan sulfate, and hyaluronate.
Explanation / Answer
1. if a mixture of lysine and aspartic acid is applied to a cation exchange column at pH=5 the first amino acid to elute would be
2. _____ is an example of a glycoaminoglycan
3. In a glycoprotein, when the carbohydrate portion is attached to a serine or threonine residue it is referred to as an ____ linked glycoprotein
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