The chymotrypsn is an enzyme used in the hydrolysis of peptides. I.e. in the cas

Question

The chymotrypsn is an enzyme used in the hydrolysis of peptides.

I.e. in the case of peptides, the KM value (the affinity to bind the substrate) is high, as a result, the rate of product formation (Vmax) increases.

Since there is a formation of acid as the result of hydrolysis of peptide (amide) bond, the enzyme is highly pH dependent.

Now, 1. The KM value decreases with other substrates.

Explanation: The other substrates may not be able to bind effectively with other substrates. As a result, the KM value is not same.

2. The Vmax value also decreases.

Explanation: As the enzyme can't effectively bnd with the other substrates, the rate of product formation will not be same.

3. The pH dependence of the enzyme does also decrease.

Explanation: As the product with other substrates may not be acid, the pH dependence of the enzyme may not be same.

Explanation / Answer

Chymotrypsin serves as a catalyst in the "Hydrolysis" of pepdite bonds - found in carboxylic groups of amino acids, which consist of aromatic pedand groups and large hydrophobic groups. Chymotrypsin is also known to serve as a catalyst in the Hydrolysis of "ester - and amide bonds" , found in hydrophobic groups.

1. Would you expect the KM of the reactions of Chymotrypsin with the different substrates to have the same value ? explain your reasoning.

2. 1. Would you expect the Vmax of the reactions of Chymotrypsin with the different substrates to have the same value ? explain your reasoning.

3. Would you expect the " pH-dependence " of the "hydrolysis - reactions" of the different substrates to be the same for all substrates ? explain your reasoning.

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