Compare the zone of inhibition chart for ampicillin (when testing Gram-positive

Question

Compare the zone of inhibition chart for ampicillin (when testing Gram-positive organisms) and chloramphenicol. What might you predict based on these data?

Chloramphenicol: 12 mm zone of inhibition
Ampicillin: 26 mm zone of inhibition

A. Ampicillin diffuses much faster/farther into the agar than does chloramphenicol. B. Most organisms are resistant to chloramphenicol but not to ampicillin. C. Most organisms are resistant to ampicillin, but not to chloramphenicol. D. Ampicillin is a much more potent antibiotic than chloramphenicol.

Explanation / Answer

the zone of inhibition of an antibiotic is the clear area around the antibiotic disc in this region there is no microbial growth because of the antibiotic. usually stronger antibiotics have larger inhibition zones because same concentration of that antibiotic is sufficient to kill the microbes and it diffuses far off as well.

therefore the correct choice should be:

Ampicillin diffuses much faster/farther into the agar than does chloramphenicol.

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