SEQUANCE: 5\'- GCU UGG GCU GCU GAU GCU CUU UAA CAU AGA GCU AAU AUU -3\' ========
ID: 70087 • Letter: S
Question
SEQUANCE:
5'- GCU UGG GCU GCU GAU GCU CUU UAA CAU AGA GCU AAU AUU -3'
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3- Convert your mRNA sequence into a DNA sequence, then make it double-stranded by writing the complementary sequence below.
4- Transcribe (write an RNA sequence) and translate (write an amino acid sequence) both the "top" and "bottom" strands starting with the first nucleotide. You must use the genetic code in your textbook or in your lecture notes for this step. DO NOT use the table above. If you encounter a stop codon, your protein sequence is done at that point.
Use the single letter designation for amino acids as given below.
Alanine (A)
Aspartic acid (D)
Asparagine (N)
Arginine (R)
Cysteine (C)
Glycine (G)
Glutamic acid (E)
Glutamine (Q)
Histidine (H)
Isoleucine (I)
Leucine (L)
Lysine (K)
Methionine (M)
Proline (P)
Phenylalanine (F)
Serine (S)
Threonine (T)
Tryptophan (W)
Tyrosine (Y)
Valine (V)
5- Use your original DNA sequence from #3. Add an A to the 5' end of the "top" strand, then write the complementary sequence on the bottom. Add a GC to the 5' end of the
"bottom" strand, then write the complementary sequence on the top. Transcribe and translate both the "top" and "bottom" with this new sequence.
What kind of mutation(s) was introduced in the "top" strand?
What kind of mutation(s) was introduced in the "bottom" strand?
6- Use your original DNA sequence from #3. Starting at the 5' end on the "top" strand of your original sequence from part 3, delete the second A, the third G, and the fourth C along with the complementary nucleotides on the bottom strand. Transcribe and translate both the "top" and "bottom" with this new sequence.
What kind of mutation(s) was introduced in the "top" strand?
What kind of mutation(s) was introduced in the "bottom" strand?
7. Use your original DNA sequence from #3. Starting at the 5' end of the "top" strand, change the third G to an A, then change the complementary nucleotide in the bottom. Starting at the 5' end of the "bottom" strand, change the third C to a T, then change the complementary nucleotide on the top. Transcribe and translate both the "top" and "bottom" with this new sequence.
a. What kind of mutation(s) was introduced in the "top" strand?
b. What kind of mutation(s) was introduced in the "bottom" strand?
Alanine (A)
Aspartic acid (D)
Asparagine (N)
Arginine (R)
Cysteine (C)
Glycine (G)
Glutamic acid (E)
Glutamine (Q)
Histidine (H)
Isoleucine (I)
Leucine (L)
Lysine (K)
Methionine (M)
Proline (P)
Phenylalanine (F)
Serine (S)
Threonine (T)
Tryptophan (W)
Tyrosine (Y)
Valine (V)
Explanation / Answer
3. mRNA sequence
5'- GCU UGG GCU GCU GAU GCU CUU UAA CAU AGA GCU AAU AUU -3'
DNA sequence:
3’- CGA ACC CGA CGA CTA CGA GAA ATT GTA TCT CGA TTA TAA - 5’
5’- GCT TGG GCT GCT GAT GCT CTT TAA CAT AGA GCT AAT ATT – 3’
4.
DNA sequence:
3’- CGA ACC CGA CGA CTA CGA GAA ATT GTA TCT CGA TTA TAA - 5’
mRNA sequence:
5'- GCU UGG GCU GCU GAU GCU CUU UAA CAU AGA GCU AAU AUU -3'
Protein sequence:
AWAADAL Stop
DNA sequence:
3’- TTA TAA TCG AGA TAC AAT CTT TCG TAG TCG TCG GGT TCG – 5’
mRNA sequence:
5’ - UUA UAA UCG AGA UAC AAU CUU UCG UAG UCG UCG GGU UCG – 3’
Protein sequence:
L stop codon SRYNLS stop codon SSGS
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