Now you study enzyme inhibition by measuring enzyme kinetics in the presence of
ID: 70153 • Letter: N
Question
Now you study enzyme inhibition by measuring enzyme kinetics in the presence of 10 mM of inhibitor A or inhibitor B (separately). The Lineweaver-Burk plots in the presence o these inhibitors arc indicated by "+A" or "+B" in the Figure below. What Type of inhibitor of A and B is shown in the figure above (Competitive, pure noncompetitive, mixed non-competitive or uncompetitive) What effect (increase, decrease, stay the same) docs the inhibitor depicted below have on Vmax What effect (increase, decrease, stay the same) docs the inhibitor depicted below have on Vmax Using the data you have (depicted in the Figure above), Determine the Km and Vmax for the uninhibited enzyme (E) and the inhibitors (A and B).Explanation / Answer
1)
Inhibitor A: competitive
Inhibitor B: mixed non-competitive
2)
Competitive inhibitor binds to the enzyme’s actives site. It affects the Km, but will not affect Vmax.
Non-competitive inhibitor lowers the Vmax.
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