Select all of the TRUE statements. (Select all that apply.) Molecular biologists
ID: 70209 • Letter: S
Question
Select all of the TRUE statements. (Select all that apply.)
Molecular biologists use restriction enzymes to amplify a specific section of DNA within the genome.
The isolation and copying of a gene, usually in large quantities is called gene cloning.
DNA ligase is needed in a cloning experiment to reestablish the stable sugar-phosphate backbone of the DNA molecule.
A vector is a small segment of DNA that a gene of interest is inserted into for cloning.
Restriction endonucleases are specific in the DNA sequences that they recognize.
Since cDNA is made from RNA, it lacks introns and contains only the coding sequence for the functional protein.
a)Molecular biologists use restriction enzymes to amplify a specific section of DNA within the genome.
b)The isolation and copying of a gene, usually in large quantities is called gene cloning.
c)DNA ligase is needed in a cloning experiment to reestablish the stable sugar-phosphate backbone of the DNA molecule.
d)A vector is a small segment of DNA that a gene of interest is inserted into for cloning.
e)Restriction endonucleases are specific in the DNA sequences that they recognize.
f)Since cDNA is made from RNA, it lacks introns and contains only the coding sequence for the functional protein.
Explanation / Answer
a)False.The first statement is false because restriction enzymes are used to cut the DNA segments but not to amplify.Polymerase chain reaction is used to amplify a specific section of DNA.
b)True.
c)True.
d)True.
e)True.
f)True.
Except the statement "a" remaining all statements are true.
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