Required Problems to be handed in: 11,12,11.13,11.16,12.5,12.7,2.8,12.10,12.12,1

Question

Required Problems to be handed in: 11,12,11.13,11.16,12.5,12.7,2.8,12.10,12.12,12.13,12.18,12.20,12.23, 12.27, 12.28, 12.29, 12.30,13.1,132,13.15,13.39. and: A. The sequence of hypothetical eukaryotic gene shown below. Only one strand of the double- stranded UNA is shown, this coding (or nontemplate) strand. Assume that the dashes represent more than one base-pair The start of transcription is the A nucleotide closest to the arrow marked +1. transcription goes toward the right, and the splice junction sequences are underlined. Assume that transcription stops at the last base given.

Explanation / Answer

a) Below are the results without splicing:

5’---CAAT------TATAA---GATGCATCATAATGGATGCGTATAGTTAGGGCATTTTAACTTAA-3’

3’—GUUA-----AUAUU---CUACGUAGUAUUACCUACGCAUAUCAAUCCCGUAAAAUUGAAUU-5’

Open reading frames are underlined.

5'3' Frame 1
V N I L R S I T Y A Y Q S R K I E

5'3' Frame 2
L I F Y V V L P T H I N P V K L N

5'3' Frame 3
Stop Y S T Stop Y Y L R I S I P Stop N Stop I

3'5' Frame 1
N S I L R D Stop Y A Stop V I L R R I L

3'5' Frame 2
I Q F Y G I D Met R R Stop Y Y V E Y Stop

3'5' Frame 3
F N F T G L I C V G N T T Stop N I N

Below are the results after splicing:

5'3' Frame 1
V N I L R S I T Y A Y I P Stop N Stop I

5'3' Frame 2
L I F Y V V L P T H I S R K I E

5'3' Frame 3
Stop Y S T Stop Y Y L R I Y P V K L N

3'5' Frame 1
N S I L R D I C V G N T T Stop N I N

3'5' Frame 2
I Q F Y G I Y A Stop V I L R R I L

3'5' Frame 3
F N F T G Y Met R R Stop Y Y V E Y Stop

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