WILDCATS Column Chromatography Final Report Mark all the component spots on your
ID: 705906 • Letter: W
Question
WILDCATS Column Chromatography Final Report Mark all the component spots on your TLC plates and tape these TLC plates into your notebook and hand them in as part of your Final Report. Be sure to calculate the theoretical yield for fluorenone, and calculate the percent yield of fluorenone, first without considering the recovered fluorene and the recalculate the percent yield taking the recovered fluorene into account. Include some discussion of your TLC results and the quality of your separation using TLC and column chromatography Questions for Final Report .Explain how each of the following would effect a chromatographic separation: (a) using too strong of an adsorbent (b) collecting elution fractions which are too large (c) the solvent level has been allowed to drop below the top of the column 2. (532 only) Write a detailed mechanism for the synthesis of fluorenone from fluorene. Be sure to include some explanation for the necessity of the phase transfer catalyst. You may need to consult your lecture text. 2.) 351 only) The formation of fluorenone from fluoren is thought to proceed via a carbanion that is formed by removal of the doubly benzylic hydrogens in fluorene followed by formation of a hydroperoxide. Draw the structures of the carbanion and the hydroperoxide.Explanation / Answer
1.(a) When Adsorbent (solid phase ) is too strong then solvent willn't rise so effreffectively on the adsorbent as there will be strong bonding and thus chromatographic separation will not be effective .
(b). The substamce to be separated is obtained at the end of the column in form of elution fractions , when these fractions are large then to separate the substance isn't easy .
c) .in this case the column will run dry and thus separation isn't effective .
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