Hi, I need some help on an immunobiology question for my class. You have develop

Question

Hi, I need some help on an immunobiology question for my class.

You have developed a mouse strain call XYZ that models the autoimmune disease pemphigus, where autoantibodies are made that destroy the tight junctions in the dermis, resulting in blisters and exfoliation, leading to life-threatening disease. As mice develop the disease over their first 24 weeks of age, the severity can be scored on a scale of zero (least severe) to five (most severe). Assume that you bred these mice to TLR7 and TLR9 knockouts, to create both single and double knockouts.

1. What conclusions do you draw about the roles of TLR7 and TLR9 in your model? Explain your reasoning.

2. What do you conclude from the double KOs? Explain your reasoning.

3. TLR9 is expressed in both B cells and T cells. Outline an experiment to detemine if the role played by TLR9 in this disease is mediated by its expression in T versus B cells.

4. Assume you discover the TLR9 effect requires its expression in T cells but not B cells. Briefly discuss the things you would like to know next, and why.

Explanation / Answer

1. From the results we can conclude that mice with the absence of TLR7 gene fail to develop the disease though the TLR9 is present (TLR9 is expressed). These results are evident from both the double knockout and single knockout groups.

2. Double knockouts are not susceptible to the development of disease, may be because of TLR7 knockout.

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