PLEASE EXPLAIN WHY TOO. You are a researcher and discover that there is a synony

Question

PLEASE EXPLAIN WHY TOO.

You are a researcher and discover that there is a synonymous point mutation in the lacO component of the Lac Operon and a frameshift mutation in the LacY component of the Lac operon. In presence of low amounts of glucose and low amounts of lactose, what is a true statement regarding this situation?

a. The Lac operon will be transcribed at very high levels due to the mutation in the LacY component.

b. The Lac operon will be transcribed at very high levels due to the mutation in the LacO component.

c. The Lac operon will be transcribed at very high levels due to the low levels of glucose.

d. The Lac operon will not be transcribed at very high levels due to the mutation in the LacO component.

e. The Lac operon will not be transcribed at very high levels due to the low levels of lactose.

f. The Lac operon will not be transcribed at very high levels due to the low levels of glucose.

Explanation / Answer

b) The Lac operon will be transcribed at very high levels due to the mutation in the LacO component.

The Lac operon is activated only in the presence of lactose. However, in this case, since there is a mutation in the O and the Y component of the lac operon, the repressor cannot bind to the operator and inhibit the Lac operon, resulting in high transcription levels of Lac operon, due to a mutation in the Lac O component.

However, a mutation in Lac Y component results in no production of lactose permease enzyme, which allows for the transport of lactose in the cell. Therefore, despite low concentrations of lactose, the Lac operon gets activated.

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